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Part:BBa_K1045011:Design

Designed by: iGEM Team Göttingen 2013   Group: iGEM13_Goettingen   (2013-09-20)


Promoter reverse


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1
    Illegal NheI site found at 24
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 55
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

To construct this part, hybridization oligos were used. These hybridization oligos encoded the sequence of BBa_J23117 and 54 additional bases upstream of this promoter. The insert generated by the hybridization of the oligos was cloned into pSB1C3 via EcoRI and SpeI.

Source

This part originated from hybridization oligos purchased from Sigma-Aldrich. The promoter sequence is that of BBa_J23117 given on its Parts Registry page, while the 54 additional bases represent a random sequence that would translate into the amino acid sequence "cyclicdiampacterim".

References